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Fig. 3 | BMC Biology

Fig. 3

From: Putative ligand binding sites of two functionally characterized bark beetle odorant receptors

Fig. 3

Functional characterization of ItypOR46. a Response of TREx/HEK293 cells expressing ItypOR46 and ItypOrco to select stimuli (30 μM) and vehicle control in the screening experiment (responses to all 68 odor stimuli are shown in Supplementary Fig. 2, Additional file 2). (+)-Induction: response of cells induced to express ItypOrco and ItypOR46; (−)-Induction: response of non-induced control cells. Asterisks (***) indicate a significant difference (at p < 0.001) between induced and non-induced cells; ns = not significant (n = 3 biological replicates, each including 3 technical replicates, i.e., ntotal = 9). b Dose-dependent responses of the same TREx/HEK293 cell line to the pure enantiomers of ipsenol and the racemate (n = 4 biological replicates, each including 3 technical replicates, i.e., ntotal = 12). EC50 values: (S)-(−)-ipsenol 1.98 μM; (±)-ipsenol 9.06 μM. c Current responses of Xenopus oocytes expressing ItypOR46 and ItypOrco in the screening experiment to the same stimuli (30 μM) as shown in a (n = 5). d Example of current trace responses from an oocyte expressing ItypOR46 and ItypOrco in the screening experiment (30 μM stimulus concentration). e Dose-dependent current response of oocytes expressing ItypOR46 and ItypOrco to racemic ipsenol (n = 6) and racemic ipsdienol (n = 5). f Dose-dependent current response of oocytes expressing ItypOR46 and ItypOrco to pure enantiomers of ipsenol (n = 4–5) and ipsdienol (n = 4). g Examples of current trace responses from oocytes expressing ItypOR46 and ItypOrco in dose-response experiments with (S)-(−)-ipsenol (left trace) and (R)-(+)-ipsenol (right trace). Data represent mean responses ± SEM (panels ac, ef)

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