Fig. 8
From: Putative ligand binding sites of two functionally characterized bark beetle odorant receptors
In situ hybridizations and responses of olfactory sensory neurons putatively associated with ItypOR46 and ItypOR49. a Detection of ItypOR46 in single-walled sensillum type 1 in antennal areas “A” and “B” in a male (left) and female (right) I. typographus as shown by whole mount in situ hybridization using a digoxigenin-labeled probe (magenta; sensilla also highlighted with yellow arrowheads). White capital letters (A, B, and C) a, indicate the three areas of sensilla [58], and ipsenol-specific olfactory sensory neurons (OSNs) are found in areas A and B [2, 29]. Electrophysiological responses of the two OSN classes tuned to b ipsenol (n = 10; 6 from males, 4 from females) and c ipsdienol (n = 4; 2 from each sex) to OR and/or OSN-active compounds and solvent blank, with representative excitatory response traces shown in d and e, respectively. Compounds were diluted in paraffin oil and presented at a high dose of 10 μg on filter paper inside standard Pasteur pipette stimulus cartridges. The black horizontal bars indicate the 0.5 s odor stimulation. OSN data in b–e originally collected by Andersson et al. [29], but re-analyzed for the purpose of the present study. For data showing the enantio-selectivity of these OSN classes, see original publications [30, 32, 33]