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Fig. 1 | BMC Biology

Fig. 1

From: Single-cell resolution landscape of equine peripheral blood mononuclear cells reveals diverse cell types including T-bet+ B cells

Fig. 1

scRNA-Seq analysis of equine PBMC from 7 horses identifies five major cell groups. a UMAP of equine PBMC (n = 34,677 total cells passing filter). Points (cells) are colored by cluster membership. Dashed outlines indicate 5 major cell groups. b Gene expression patterns informing major cell group assignments. Expression values are scaled independently for each plot, ranging from 2.5 to 97.5 percentile of gene expression across all cells. Gene ID ENSECAG00000000419 is labeled as T Cell Receptor Alpha Chain C Region based on Ensembl/NCBI annotations. c Heatmap of differentially expressed genes (adjusted p value < 0.05, log2 fold-change > 1 for each major cell group versus all other major cell groups, expressed > 25% of cluster). For each major cell group, 30 cells (columns) were randomly selected from each horse for plotting purposes. d Frequency of each major cell group in total PBMC per horse

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