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Fig. 3 | BMC Biology

Fig. 3

From: Single-cell resolution landscape of equine peripheral blood mononuclear cells reveals diverse cell types including T-bet+ B cells

Fig. 3

Equine peripheral B cell clusters include several distinct transcriptional states marked by expression of different transcription factors, including T-bet+ B cells. Clusters within the B cell major cell group were further analyzed and annotated by differential gene expression. Cluster 25 was excluded from analysis due to low transcript counts. a UMAP subset of B cell clusters with putative cluster annotations. b Frequency of each cell cluster within the B cell group per horse. c Hierarchical clustering (integrated PCA dimensions) of B cell major cell group. d Heatmap of differentially expressed genes (adjusted p value < 0.05, log2 fold-change > 1 for each cluster versus all other clusters, expressed > 25% of cluster). e Dot plot of select transcription factor genes differentially expressed across B cell clusters. Dot size is proportional to number of cells with detectable expression of indicated gene. Dot color intensity indicates average gene expression values scaled across plotted clusters. *Gene ID ENSECAG00000029287 is labeled HOPX based on Ensembl/NCBI annotation. f Violin plot of immunoglobulin heavychain isotype transcript expression for indicated B cell clusters. Expression values are log-normalized per cell. g Hierarchical clustering of equine PBMC scRNA-Seq data (B cell clusters) and human PBMC scRNA-Seq data (B cell clusters). Median-normalized average expression values for highly variable human/horse one-to-one orthologs were calculated for each cluster, and clustering was performed on Pearson distances by Ward’s method

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