Fig. 4

Equine T-bet⁺ B cells exhibit gene expression and cell surface protein marker expression characteristic of human T-bet⁺ B cells and express diverse surface immunoglobulin isotypes. a Expression patterns for select genes associated with T-bet⁺ atypical B cells in humans and/or mice. Expression values are scaled independently for each plot, ranging from 2.5 to 97.5 percentile of gene expression across all B cell clusters. *Gene ID ENSECAG00000031055 labeled as CR2 (CD21) based on Ensembl/NCBI annotation. b Flow cytometry gating strategy and expression of surface markers (PanIg and CD11b) and T-bet. c Expression of surface markers CD21 and CD23 in T-bet⁺ B cells. d Expression of surface immunoglobulin isotypes (IgM, IgG1) in T-bet⁺ B cells. e Quantification of surface immunoglobulin isotype expression by flow cytometry. f Correlation of T-bet⁺ B cells as determined by scRNA-Seq compared to flow cytometry. Pearson correlation coefficient, r = 0.93