Fig. 4
From: Control of glutamate release by complexes of adenosine and cannabinoid receptors
A2AR C-terminus-guided homomeric interface of the CB1R homodimer in the A2AR-CB1R heterotetramer. a–c BiFC experiments with synthetic peptides with the amino acid sequence of the TMs of CB1R and A1R. HEK-293T cells were transiently transfected with the following: a cDNA of two different molecules of CB1R separately fused to complementary halves of YFP (CB1R-cYFP and CB1R-nYFP; 3 μg in both cases) with co-transfection with cDNA of non-fused A2AR mutant lacking the C-terminal end (A2AΔCTR; 1 μg); b, c cDNAs of two different molecules of A1R separately fused to complementary halves of YFP (A1R-cYFP and A1R-nYFP; 3 μg in both cases) with co-transfection with cDNA of non-fused A2AR (b; 1 μg) or non-fused A2AΔCTR (c; 1 μg). Cells were treated for 4 h with medium (control) or 4 μM of indicated TM peptides (numbered 1–7). Values are means ± S.E.M. (n = 6 with triplicates in all the experiments) of the percentage of the fluorescence in the control group and analyzed statistically with repeated measures ANOVA, followed by Dunnett’s multiple comparison test (***: p < 0.001, compared with control)