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Fig. 2 | BMC Biology

Fig. 2

From: The mitochondrial HSP90 paralog TRAP1 forms an OXPHOS-regulated tetramer and is involved in mitochondrial metabolic homeostasis

Fig. 2

Absolute quantitation of traced metabolites in WT and KO cells. a Quantitation of total glutamine and glutamate levels. b Quantitation of TCA cycle metabolites. c Sensitivity of WT and KO HEK293T cells to the glutaminase inhibitor CB-839, see the “Materials and methods” section for experimental details. The error bars represent the standard error of the mean for three independent experiments. d Quantitation of reduced (GSH) and oxidized glutathione (GSSG) in WT and KO HEK293T and A549 cells. The absolute quantitation shown is for metabolites with increased 13C abundance from labeled glutamine (see data in Additional file 4: Table S2). Data points on bar graphs of a, b, and d indicate metabolite concentration per 106 cells from each biological replicate (n = 2)

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