Fig. 3

Analysis of the TRAP1 structure activity relationship for metabolic regulation. a OCR traces of WT versus KO HEK293T cells exogenously expressing the control proteins mitoEGFP or WT TRAP1, or the TRAP1 truncation mutants MTS-N, MTS-M, and MTS-C. b Quantitation of the basal respiration rates of WT versus KO HEK293T cells expressing the indicated proteins. c OCR traces; experiments as in a, but with the TRAP1 truncation mutants MTS-N+M and MTS-M+C. d Quantitation of the basal respiration rates of WT versus KO cells expressing the indicated proteins. e–h OCR traces of WT versus KO HEK293T cells overexpressing WT TRAP1 (e), the ATPase mutants E115A/R402A (f), ΔSTRAP (g), or D158N (h). i Quantitation of the basal respiration rates of WT versus KO HEK293T cells expressing the indicated proteins. ns, difference statistically not significant. j OCR traces with WT and KO MAFs and MAF KO cells exogenously expressing either WT TRAP1 or the TRAP1 low ATPase mutant E115A. All data are reported as means ± SEM (n = 3) with asterisks indicating statistically significant differences between the compared groups (*p < 0.05, **p < 0.01, and ***p < 0.001)