Fig. 7

TRAP1 complexes with inhibition and induction of OXPHOS. a Immunoblot of a native gel analysis of TRAP1 complexes from MCF-7 cells upon inhibition of OXPHOS at different steps. The lower panel shows the quantitation (dotted green lines only serve to indicate the baseline without treatment). b Immunoblot of a native gel analysis of TRAP1 complexes from HEK293T cells upon inhibition of OXPHOS at different steps alone and in combination. c ECAR profiles of HEK293T cells treated with OXPHOS inhibitors (ORA, cocktail of oligomycin, rotenone, and antimycin) with or without an LDH inhibitor (LDHi, 5 μM). d OCR profile of HEK293T cells treated with OXPHOS inhibitors (ORA, cocktail of oligomycin, rotenone, and antimycin) with or without an LDH inhibitor (LDHi, 5 μM). e Immunoblot of a native gel analysis of TRAP1 complexes from HEK293T cells treated with LDHi for 2, 4, and 6 h