Fig. 5

Composition of the A. godoyi mitochondrial ribosome. The figure lists the mtRPs inferred to have been present in the mitoribosome of LECA [114]. The blue-shaded ovals encompass those mtRPs directly inherited from the alphaproteobacterial ancestor of mitochondria (those shown in orange are mtDNA-encoded in A. godoyi, black are nuclear DNA-encoded). Proteins shown in blue italics are eukaryote-specific additions. Proteins set in square brackets are present in both A. castellanii and N. gruberi but were not retrieved from A. godoyi genomic or transcriptomic data, despite extensive searches. Three components (strikethrough) have subsequently been shown to be absent in the cryo-EM structure of the yeast mitoribosome [115], with S36 having another, non-ribosomal function [116]. We identified Andalucia homologs of these three proteins, with S26 and Ppe1 predicted to be mitochondrion-targeted