Fig. 3

Optimal combination of the cell-specific promoters increases the number of identified cells in an animal. a Number of positive cells and stability of expression of cell-specific promoters. A cell was counted as positive for a promoter if the cell expresses the landmark fluorescent protein in at least one animal. The positive ratio is a ratio of positive (expressing) cells over the total number of the cells (= number of tested animals). Stability of expression was calculated as an average of the positive ratio over the cells in which at least one cell is positive for the promoter. b Number of positive cells and sparseness of the expression pattern (see the “Methods” section) of the cell-specific promoters. Note that, for visibility, only the several labels are shown in a and b. Fully labeled panels are shown in Additional file 12: Figure S8. c Visualization of the optimal combination of the cell-specific promoters. The cells in the right half of the body are shown. d A part of c is zoomed for comparison with e. e An example fluorescent image of JN3039 strain and annotated cell names. See Additional file 14: Figure S9 for enlarged image. f The number of the detected and the identified nuclei in each animal. g The names of identified cells and the identification ratio, which is a ratio of identified cells over the total number of the cells. The solid lines are guide for visualization