Table 1 Strain list used in this study

Bristol N2

C. elegans wild isolate

Figure S10

JN3000

Ex[casy-1p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3001

Ex[ceh-10p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3002

Ex[daf-28p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3003

Ex[daf-7p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3004

Ex[dat-1p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3005

Ex[dyf-11p::nls4::YFP, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3006

Ex[eat-4p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3007

Ex[eat-4p::svnls2::TagRFPsyn;lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3008

Ex[flp-6p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3009

Ex[flp-7p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3010

Ex[flp-12p::nls::Venus, lin-44p::mCherry]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3011

Ex[gcy-22p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3012

Ex[gcy-28p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3013

Ex[gcy-7p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3014

Ex[glr-1p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3015

Ex[glr-2p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3016

Ex[glr-3p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3017

Ex[gpa-2p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3018

Ex[gpa-10p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3019

Ex[gpa-13p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3020

Ex[gpc-1p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3021

Ex[lim-4p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3022

Ex[ins-1::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3023

Ex[tdc-1::mTFP, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3024

Ex[ins-1(short)p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3025

Ex[mbr-1p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3026

Ex[nep-2sp::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3027

Ex[npr-9p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3028

Ex[odr-2p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3029

Ex[sdf-9p::SDF9::GFP, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3030

Ex[sdf-9p::nls::GFP, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3031

Ex[ser-1p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3032

Ex[ser-2(prom2)p::mTFP, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3033

Ex[sra-6p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN2101

Ex[tax-4p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3035

Ex[ttx-3p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3036

Ex[vem-1p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 1, 2, 3, and 4

JN3038

qjIs11[glr-1p::svnls2::TagBFPsyn, ser-2(prom2)p::svnls2::TagBFPsyn]; peIs3042[eat-4p::svnls2::TagRFP675syn, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry]; qjIs14[H20p::nls::YC2.60].

Figs. 3, 4, and S10

JN3039

qjIs11[glr-1p::svnls2::TagBFPsyn, ser-2(prom2)p::svnls2::TagBFPsyn]; peIs3042[eat-4p::svnls2::TagRFP675syn, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Figs. 3, 4, and S10

JN3041

Ex[ptr-10p::nls::YC2.60, lin-44p::GFP]; peIs2100[H20p::nls4::mCherry].

Fig. 1

ZIM945

lite-1(xu-7); mzmIs4[Punc-31::NLSGCaMP5K; Punc-122::gfp]

Figure S10

ZIM1048

lite-1(ce314); mzmIs4[Punc-31::NLSGCaMP5K; Punc-122::gfp]

Figure S10