Fig. 4
Decreased Tor, or increased Atg5 to increase autophagy, suppresses Adar5G1 mutant phenotypes. a Rescue of Adar5G1 mutant open field locomotion defects in Adar5G1; TorK170048/ +, Adar5G1; TorMB07988/ +, Adar5G1; ChAT>Atg5, and Adar5G1; ChAT>Atg1 flies but not in Adar5G1; ChAT>Thor or Adar5G1; ChAT>S6KKD and very partially in Adar5G1; ChAT>TSC1,TSC2 flies. n > 8. b Representative images of MB calyx (× 63) and c retina (× 40) in 30-day Adar5G1; ChAT>Atg5. Scale bars, 20 μm. d Immunoblot with antibody to Synaptotagmin 1 of head protein extracts of Adar5G1, w1118, Adar5G1; TorK17004 / +, and Adar5G1; ChAT > Atg5 flies. Quantitation of immunoblot data shows increased Synaptotagmin 1 in Adar5G1 is reduced by decreased Tor or by increased Atg5. n ≤ 3. e Immunoblot with antibody to ref(2)p, the Drosophila p62 canonical autophagy protein, of head protein extracts of w1118, Adar5G1mutant, Adar5G1; TorK17004 / +, and Adar5G1; ChAT > Atg5 flies. Quantitation of immunoblot data shows that increased ref(2)p, Drosophila p62 protein, in Adar5G1 is not reduced but increased by decreasing Tor or by increasing Atg5. n ≥ 3. p values were calculated by a one-way ANOVA followed by Tukey’s test. The significance of differences between variables was described based on p values: *p value < 0.05, **p value < 0.005, ***p value < 0.001, and n.s (not significant). Error bars: SEM (standard error of mean for biological replicates). Source data values are included in Additional file 6