Fig. 5

ADAR protein expression rescues the autophagy-related phenotype in Adar^5G1 larval fat cells. The fat bodies of a–c wild-type strain w¹¹¹⁸, d–fAdar^5G1;CgIV>, and g–iAdar^5G1;CgIV>UAS-Adar3/4 have been dissected and live-stained with DAPI (a, d, g) and Lysotracker (b, e, h) dyes (merges in c, f, i). Wild-type fat body does not show any Lysotracker staining (b, c). Adar^5G1 mutant fat cells have an increased activation of autophagy as indicated by increased Lysotracker staining in lysosomes (e, f). Expression of the UAS-Adar3/4 transgene in the Adar^5G1 mutant fat cells is sufficient to rescue the elevated basal autophagy (h, i). Scale bars, 50 μm