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Table 1 Studies analyzed

From: Contaminant DNA in bacterial sequencing experiments is a major source of false genetic variability

Study name

Publication

Runs analyzed

Sample source

Dataset

Mozambique

Unpublished

138

Clinical isolates

MTB dataset

Kwazulu-Natal

Cohen et al. 2015

433

Single colonies from clinical isolates

MTB dataset

Nigeria

Senghore et al. 2017

73

Clinical isolates

MTB dataset

Belarus

Wollenberg et al. 2017

552

Clinical isolates

MTB dataset

High-depth sequencing

Trauner et al. 2017

63

Clinical isolates

MTB dataset

Sputum-capture sequencing

Brown et al. 2015

58

Clinical respiratory specimens (culture-free sequencing with a bait capture strategy)

MTB dataset

Sputum-direct sequencing

Votintseva et al. 2017

68

Clinical respiratory specimens (direct culture-free sequencing)

MTB dataset

MGIT sequencing*

Pankhurst et al. 2016

168

Early-positive MGIT cultures (liquid)

MTB dataset

A. baumannii

Willems et al. 2016

36

Single-colony recultured in broth

Bacterial dataset

C. difficile

Stone et al. 2016

54

Pooled single-colony isolates

Bacterial dataset

Enterococcusā€ 

Tyson et al. 2018

197

Isolates from retail meats

Bacterial dataset

K. pneumoniae

Holt et al. 2015

285

Human, animal, and environmental isolates

Bacterial dataset

L. monocytogenes

Halbedel et al. 2018

424

Clinical isolates from human

Bacterial dataset

L. pneumophila

Timms et al. 2018

48

Pure culture isolates from human and cooling towers

Bacterial dataset

N. gonorrhoeae

Yahara et al. 2018

272

Pure culture isolates from human

Bacterial dataset

P. aeruginosa

Marvig et al. 2015

445

Clinical isolates from human

Bacterial dataset

S. aureus

Aanensen et al. 2016

337

Clinical isolates from 186 hospitals in 21 countries

Bacterial dataset

S. enterica

Gymoese et al. 2017

366

Human, animal, and environmental isolates

Bacterial dataset

T. pallidum

Pinto et al. 2016

25

Clinical specimens (culture-free sequencing with a bait capture strategy)

Bacterial dataset

Vibrioā€”

Greig et al. 2018

152

Clinical isolates from human

Bacterial dataset

  1. *This study included sequencing samples from non-MTB organisms. We analyzed the 168 reported as MTB by the authors
  2. ā€ This study included sequencing samples from 2 species (E. faecalis and E. faecium)
  3. ā€”This study included sequencing samples from different Vibrio species. We only analyzed the 152 reported as V. cholerae by the authors