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Fig. 6 | BMC Biology

Fig. 6

From: Ca2+ mobilization-dependent reduction of the endoplasmic reticulum lumen is due to influx of cytosolic glutathione

Fig. 6

GSH transport can be triggered by cyclosporine A. a Real-time fluorescence ratio changes of Grx1-roGFP1-iEER in response to 10 micromolar CsA in HEK293 cells stably expressing the sensor. Each trace corresponds to the data recorded from one cell; traces were obtained from two independent experiments. At the end of each experiment, 500 μM diamide (Dia) and 20 mM DTT were added to ensure the functionality of the probe. Cells were left untreated or treated overnight with 1 mM BSO prior to the experiment. b, c Experiment performed as in a, but 50 μM FK506 (b) or 10 μM cyphermethrin (c) was applied as marked instead of CsA. d HEK293 cells stably expressing Grx1-roGFP1-iEER were transfected with control, cyclophillin A or B siRNA for 48 h before imaging; 10 μM CsA were applied as indicated by the arrow. Knockdown efficiency was verified by qPCR

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