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Fig. 7 | BMC Biology

Fig. 7

From: Ca2+ mobilization-dependent reduction of the endoplasmic reticulum lumen is due to influx of cytosolic glutathione

Fig. 7

The Sec61 translocon polypeptide channel does not participate in glutathione transport. Effects of manipulating the translocon on fluorescence ratio changes of Grx1-roGFP1-iEER in HEK293 cells stably expressing the sensor. Each trace corresponds to the data recorded from one cell. At the end of each experiment, 500 μM diamide (Dia) and 20 mM DTT were added to ensure the functionality of the probe. a One hundred micromolar puromycin, b 200 μM anisomycin followed by 100 μM puromycin, e 200 μM anisomycin followed by 1 μM TG, and f 100 μM puromycin followed by 1 μM TG were applied as indicated by the arrow. c Cells were treated overnight with 1 mM BSO prior to experiment, and 100 μM puromycin were applied as marked. d HEK293 cells stably expressing Grx1-roGFP1-iEER were transfected with control or Sec61 siRNA for 48 h before imaging as above; 1 μM TG were applied as indicated by the arrow. Knockdown efficiency was verified by Western blot (aSec61a, anti-Sec61α antibody; aBiP, anti-BiP antibody; aActin, anti-actin antibody)

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