Fig. 8
Calreticulin is dispensable for ER reduction induced by Ca2+ depletion or Cyclosporin A. Wild-type and CRT −/− mouse embryonic fibroblasts were transfected with Grx1-roGFP1-iEER, and real-time fluorescent ratio changes were monitored. Reductive shift-provoking agents were applied as indicated. Each trace represents the data recorded from one cell; traces shown are representative of three independent experiments