Fig. 1.

drxIR1 locus causes increased polyQ40 aggregation. a Late-L4 RIL2 and drxlR1;Q40 animals have increased aggregation compared to Q40Bristol animals. Insets show polyQ40 aggregation in the head muscles. b The scheme for generation of the drxIR1;Q40 strain through rounds of serial backcrossing/selection. RIL2 strain was backcrossed (BC) into the Q40Bristol strain 23 times. DR1350-derived variants (red) that are retained through the crossing-selection scheme likely contribute to the RIL2 polyQ phenotype. c The drxIR1;Q40 animals exhibit a faster accumulation of polyQ aggregates compared to Q40Bristol at all development stages, until both strains reach maximum at day 2 of adulthood. L3, L4, YA, and D2 adult indicate third and fourth larval stage, young adult, and day 2 adult stage, respectively. Data are mean ± SD, 10 to 20 animals per data point. Data were analyzed by ANOVA followed by Bonferroni’s multiple comparisons test, ****P < 0.0001, ***P = 0.0004. Orange: Q40Bristol background, red: drxIR1;Q40. Same color scheme is used in all figures. d Distribution of DR1350-derived SNPs and any de novo mutations on chromosome I that distinguish drxIR1;Q40 from Q40Bristol and Hawaiian strains. Gray-shaded area to the left of unc-11 shows a locus with over 3000 unique SNPs in drxIR1;Q40 strain