Fig. 2.

Basal protein homeostasis of muscle cells is unaffected in animals carrying the drxIR1 interval. a Expression of GFP::UNC-54 fusion protein from unc-54 promoter is similar between the Bristol and drxIR1 L4 animals. Data are mean ± SD of GFP fluorescence intensity, 16–20 muscle cells per genotype, unpaired t test, two-tailed. b Myofilament assembly is normal in drxIR1 animals. Confocal images of muscle cells. Scale bar, 10 μm. c Muscle cells have very few GFP::LGG-1-positive puncta (arrowheads) in both Bristol and drxIR1 L4 animals. One muscle quadrant is shown between punctate lines. m, muscle; hyp, hypodermis. An increased number of GFP::LGG-1-positive puncta is seen in the hypodermis of drxIR1. Scale bar is 10 μm. Right panel, quantification of GFP::LGG-1 puncta in the muscle cells. Data are mean ± SD, 30 to 40 cells (8 to 10 animals) per genotype, unpaired t test, two-tailed; each symbol represents individual cell. d No difference in the average intensity of the proteasome reporter fluorescence in Q40Bristol and drxIR1;Q40 animals. Data are mean ± SD, 4–5 animals, unpaired t test, two-tailed. e The increased aggregation phenotype in animals carrying the drxIR1 interval does not depend on DAF-16 or HSF-1. Each symbol represents an individual animal, 15 mid-L4 animals per genotype. O/E, overexpression. Means ± SD are overlaid. Data were analyzed by ANOVA followed by Bonferroni’s multiple comparisons test, ****P < 0.0001