Skip to main content
Fig. 5. | BMC Biology

Fig. 5.

From: Unexpected cell type-dependent effects of autophagy on polyglutamine aggregation revealed by natural genetic variation in C. elegans

Fig. 5.

Activation of autophagy has divergent effects on polyQ40 aggregate clearance in different tissues. a Animals carrying the drxIR1 interval accumulate more GFP::LGG-1-positive puncta (arrowheads) in the body-wall muscle cells upon treatment with autophagy-activating drug ABT-737. Animals were treated with 0.1% DMSO (vehicle control) or 10 μM ABT-737 for 24 h. Shown are confocal projections; one muscle quadrant (m) is indicated between punctate lines. Scale bar, 10 μm. b Autophagy-activating drug ABT-737 increases polyQ40 aggregation in the body-wall muscle cells in the wild-type background (Q40Bristol). Aggregation was scored in adult animals, 1 day post-L4 (see the “Methods” section). Aggregation in the drxIR1;Q40 animals is already at maximum under these conditions. Each symbol indicates an individual animal; overlaid are means ± SD. Data were analyzed by ANOVA followed by Bonferroni’s multiple comparisons test, ***P = 0.0006. c Activation of autophagy with mlst-8 RNAi increases aggregation in the body-wall muscles of Q40Bristol mid- or late-L4 animals, and of drxIR1;Q40 mid-L4 animals. Data are mean ± SD, 3 independent experiments, 9 to 13 animals per experiment per treatment. Control RNAi was mec-4. Data were analyzed by ANOVA followed by Bonferroni’s multiple comparisons test, ***P = 0.0007, **P = 0.0082. d Introduction of the daf-2(e1370) allele increases polyQ40 aggregation in the body-wall muscles in both Q40Bristol and drxIR1;Q40 animals. Aggregation was scored at mid-L4. Each symbol indicates an individual animal; overlaid are means ± SD. Colors as in b. Data were analyzed by ANOVA with Bonferroni’s multiple comparisons test, ****P < 0.0001. e Activation of autophagy with mlst-8 RNAi strongly suppresses polyQ aggregation in the intestinal cells. Percent of animals with Q44::YFP aggregates in the intestine of day 4 adult were scored, as in refs. [76, 77], for each indicated RNAi treatment. Control RNAi was mec-4. Data are mean ± SD. Data were analyzed by ANOVA followed by Bonferroni’s multiple comparisons test, ***P = 0.0003. f The drxIR1 interval decreases accumulation of polyQ67 aggregates in the neurites of head neurons. Aggregation was scored in day 1 adults over the dendritic area in the head, as shown in Additional file 4: Figure S4. Each symbol indicates an individual animal; overlaid are means ± SD. Data were analyzed by an unpaired t test, two-tailed, *P = 0.0332

Back to article page