Fig. 1

3D imaging techniques for life sciences applications, shown according to their spatial resolution (in XY) and the full depth (in Z) of the volume that can be imaged (accumulated over many serial sections for destructive methods). Blue = CT techniques, green = electron microscopy techniques, pink = light microscopy techniques. Solid line = non-invasive, dashed line = destructive. TEM serial section transmission electron microscopy, SEM serial section scanning electron microscopy, Soft nCT soft nano-computed tomography, Hard nCT hard nano-computed tomography, μCT micro-computed tomography. Data from [1,2,3,4,5]