Fig. 2
From: A metabolic switch regulates the transition between growth and diapause in C. elegans
DAF-16 determines the energy expenditure and lifespan of dauer larvae and, together with DAF-12, the switch to gluconeogenesis. a Cumulative heat dissipation of developmentally arrested daf-2 dauers and daf-2;daf-12 L3 larvae. Median heat of biological replicates from one experiment. Biological replicates from various experiments are displayed in Additional file 3: Fig. S3a. b Formation of dauer-like larvae of daf-16 on 4-MS. DAF-12 is activated due to a lack of DAs; however, DAF-16 activity is absent due to the mutation in the daf-16 locus. c Cumulative heat dissipation of developmentally arrested wild-type (N2) dauers and daf-16 dauer-like larvae on 4-MS. Median heat of biological replicates from one experiment. Biological replicates from various experiments are displayed in Additional file 3: Fig. S3b. d Coherent anti-Stokes Raman scattering (CARS) microscopy of lipid droplets in daf-2 dauers and daf-2;daf-12 arrested L3 larvae grown at 25 °C, and wild-type (N2) dauers and daf-16 dauer-like larvae grown on 4-MS. SHG, second harmonic generation. Representative images of at least 6 animals, scale bars 10 μm. e Survival rates of daf-2 dauers and daf-2;daf-12 arrested L3 larvae grown at 25 °C, and wild-type (N2) dauers and daf-16 dauer-like larvae grown on 4-MS. Means ± 95% confidence intervals of 3 experiments with 3 replicates. ***p < 0.001; ns, no significant difference determined by log-rank test. f 2D-TLC of 14C-acetate-labeled metabolites from daf-2 dauers and daf-2;daf-12 arrested larvae grown at 25 °C compared to L3 larvae grown at 15 °C. Representative images from at least 2 experiments. g 2D-TLC of 14C-acetate-labeled metabolites from wild-type (N2) dauers and daf-16 dauer-like larvae on 4-MS compared to L3 larvae on cholesterol. Representative images from at least 2 experiments. f, g Tre, trehalose; Glc, glucose; Glu, glutamate; Gly, glycine; Ser, serine; Gln, glutamine; Ala, alanine; Thr, threonine