Fig. 5
From: A metabolic switch regulates the transition between growth and diapause in C. elegans
DAF-12 and AAK-2 control the molar ratios of the enzymes at the branch points between competing pathways. a Scheme of the first branch point—the entry of isocitrate into glyoxylate or TCA cycle. b Molar ratio between ICL-1 and the summed abundances of all isocitrate dehydrogenase isoforms and subunits, IDHA-1, IDHB-1, IDHG-1, IDHG-2, IDH-1, IDH-2, dubbed IDH (total). c Molar ratios between ICL-1 and individual isocitrate dehydrogenases. The sums of IDHG-1+IDHG-2 and IDH-1+IDH-2 are provided as a clearer representation due to the low molar abundance of IDHG-2 and IDH-2 compared to IDHG-1 and IDH-1, respectively. Lines between data points are provided for better visualization. d Scheme of the second branch point—the recycling of oxaloacetate into citrate or its entry into gluconeogenesis. e Molar ratio between CTS-1 and the summed abundances of the two PEPCK isoforms PCK-1 and PCK-2. f Molar ratio between CTS-1 and the individual PEPCK isoforms. Lines between data points are provided for better visualization. In all panels, means ± SD of 3 biological replicates with 2 technical replicates each; p values represent p > 0.05 (ns), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. One-way ANOVA was performed with Holm-Bonferroni statistical method