Fig. 4

TADE suppression drive. a The target gene of a TADE suppression drive is at a different site from the drive allele (modeled as an unlinked site), which is located in a female (or male) fertility gene. The drive disrupts the fertility gene, so female drive homozygotes are sterile (“drive homozygote fitness” does not apply). Germline activity disrupts the target gene, and the nuclease promoter is selected to minimize embryo activity. The target gene is haplolethal, so any individuals inheriting fewer than two wild-type target alleles and/or drive alleles are nonviable. b The genetic load imposed by a TADE suppression drive in our deterministic model. If the germline cleavage rate is 100%, eradication will occur. Otherwise, eradication will only occur if the genetic load can overcome the fitness advantage of individuals at low population density. Note that this drive loses the ability to increase in frequency in any population when the germline cut rate is very low. c The speed at which the TADE suppression drive reaches 99% of individuals in the population with varying introduction frequency and drive fitness. Full suppression or an equilibrium state will be attained within a few generations of this point. The dashed line indicates the introduction frequency threshold in the deterministic model. d Same as c, but with varying germline and embryo cleavage rate