Fig. 1

The rRF analysis pipeline reveals many unique fragments. a Workflow of the pipeline. b Table showing the number of unique rRFs that map to each rRNA and the number of rRFs per unit length, for the 434 LCL samples. The rRNAs were padded with 50 nts on each side. Note that many rRFs have abundance ≥ 10 RPM. c Distribution of the number of LCL samples (out of 434) in which a given isomiR, tRF, or rRF could be found at an abundance ≥ 10 RPM. The boxplots are grouped by genome of origin, nuclear or mitochondrial. d Boxplots show the distribution of the average abundance (in RPM) for molecules belonging to each category. Only molecules with abundance ≥ 10 RPM were considered in our subsequent analysis. Boxplots are grouped by genome of origin. c, d The width of the boxes is proportional to the number of unique molecules in each category, and the horizontal bars in each box represent the median RPM