Fig. 5

Growth assays of lipoylation deficient strains complemented with LplA. All plasmids transformed into the different strains are based on the YEp352 yeast episomal plasmid backbone. a Δlip2, Δgcv3, and Δlip3 strains complemented with YEp352-LplA and YEp352-LplAK133L as a negative control. Cells were grown for 16 h, adjusted to an OD₆₀₀ of 0.5 and spotted as 1× 1/10×, 1/100×, and 1/1000× serial dilutions on SCD-URA media as a general growth control, and on SCG-URA media supplemented with LA or C8. Plates were incubated at 30 °C for 4 days. b Δhtd2Δlip2, Δhtd2Δgcv3, and Δhtd2Δlip3 complemented with YEp352-LplA, or YEp352-LplAK133L as a negative control, were spotted on a SCD-URA plate as a general growth control and on SCG-URA media with or without LA or C8. Plates were incubated at 30 °C for 4 days