Fig. 6
From: Genetic dissection of the mitochondrial lipoylation pathway in yeast
Western blot analysis of Δlip2, Δgcv3 and Δlip3 yeast strains complemented with LplA. All plasmids transformed into the different strains are based on the YEp352 yeast episomal plasmid backbone. Whole cell extracts were collected from cells expressing either YEp352-LplA, or YEp352-LplAK133L as a negative control, after 16 h of growth at 30 °C on YPG media. Blots were analyzed with anti-LA serum, and anti-actin serum as a loading control. a Whole cell extracts from wild-type+YEp352, Δlip2 + YEp352-LplA, and Δlip2 + YEp352-LplAK133L grown without supplements (−), or with 100 μM LA or 100 μM C8, were analyzed with anti-LA serum. b Whole cell extracts from wild-type+Yep352, Δgcv3 + YEp352-LplA and Δgcv3 + YEp352-LplAK133L grown without supplements (−), with 100 μM LA or 100 μM C8 analyzed with anti-LA serum. c Whole cell extracts from wild-type+YEp352, Δlip3 + YEp352-LplA, and Δlip3 + YEp352-LplAK133L grown without supplements (−), or with 100 μM LA or 100 μM C8 were analyzed with anti-LA serum