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Fig. 7 | BMC Biology

Fig. 7

From: Evolutionary dynamics and structural consequences of de novo beneficial mutations and mutant lineages arising in a constant environment

Fig. 7

Recurrent mutations in global regulator rho. a Location and frequency of mutations along the primary structure. Circles represent alleles from chemostat 1, triangles represent alleles from chemostat 2, and squares represent alleles from chemostat 3. Scale bar (0–100) indicates frequency attained by a particular mutant in an experimental population. The N-terminal primary RNA-binding domain (aa 22-116) is shown in stipple. P-loop (aa 179-183), Q-loop (aa 278-290), and R-loop (aa 322-326) residues are indicated with corresponding letters. b Rho allele frequencies over time for chemostats 1, 2, and 3. c Crystal structure of E. coli Rho (PDB ID 1PVO) showing the location of high-frequency mutations in panel b. Subunits A–F are depicted counterclockwise from the upper right. Val278 is shown in green, Ala293 in cyan, Arg87 in red, and Q-loop residues in yellow stick representation. d Detail view ribbon representation of a single Rho subunit (PDB ID 2HT1) with Val278, Ala293, Arg87, and Q-loop residues colored as in panel c

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