Fig. 2

Aging affects HSPC clusters differently. a UMAP plot (same as in Fig. 1b) showing the young (orange) and the aged (purple) HSPCs. b Distribution of young (orange) and aged (purple) HSPCs in the clusters. Left panel presents percentages of young and aged HSPCs in primed/non-primed clusters gathered and in each of the 15 clusters. The black vertical line indicates expected young and aged cell proportions according to dataset size. Names of the clusters for which proportion of aged or young cells was significantly higher than expected (hypergeometric test p value < 0.05) are colored in purple for aged cells and in orange for young cells. On the right, barplots represent the number of cells composing each ensemble: primed/non primed clusters gathered and individual clusters. c Absolute number per mouse of young and aged sorted HSPCs into the 15 groups