TY - JOUR AU - Hof, Lotta AU - Moreth, Till AU - Koch, Michael AU - Liebisch, Tim AU - Kurtz, Marina AU - Tarnick, Julia AU - Lissek, Susanna M. AU - Verstegen, Monique M. A. AU - van der Laan, Luc J. W. AU - Huch, Meritxell AU - Matthäus, Franziska AU - Stelzer, Ernst H. K. AU - Pampaloni, Francesco PY - 2021 DA - 2021/02/24 TI - Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis JO - BMC Biology SP - 37 VL - 19 IS - 1 AB - Organoids are morphologically heterogeneous three-dimensional cell culture systems and serve as an ideal model for understanding the principles of collective cell behaviour in mammalian organs during development, homeostasis, regeneration, and pathogenesis. To investigate the underlying cell organisation principles of organoids, we imaged hundreds of pancreas and cholangiocarcinoma organoids in parallel using light sheet and bright-field microscopy for up to 7 days. SN - 1741-7007 UR - https://doi.org/10.1186/s12915-021-00958-w DO - 10.1186/s12915-021-00958-w ID - Hof2021 ER -