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Fig. 1 | BMC Biology

Fig. 1

From: Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Fig. 1

Time-resolved live LSFM recordings for detailed qualitative inspections of dynamic morphological processes in organoid development. hCCAOs and mPOs were seeded into Z1-FEP-cuvettes for long-term live observations. They expressed the nuclei marker H2B-eGFP (magenta) or Rosa26-nTnG (grey) and the F-actin cytoskeletal marker LifeAct-mCherry (green). About 120 organoids were recorded in image stacks up to 900 z-planes deep for at most 7 days. The figure shows excerpts of maximum intensity z-projections. Microscope: Zeiss Lightsheet Z.1; objective lenses: detection: W Plan-Apochromat × 20/1.0, illumination: Zeiss LSFM × 10/0.2; laser lines: 488 nm, 561 nm; filters: laser block filter (LBF) 405/488/561; voxel size: 1.02 × 1.02 × 2.00 μm3; recording interval: 30 min; scale bars: 50 μm, 25 μm (inset)

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