Fig. 6
The C-terminal part of BCLA is immunogenic and drives a specific humoral response in mice only over the chronic phase of infection by cystogenic T. gondii strains. Single Western blot strips were loaded with 0.5 μg of recombinant rBCLA. The strips were tested on the sera collected from mice in the acute and chronic phases of toxoplasmosis with various T. gondii strains, route of infection, and mouse genetic background. a IgG immunoblot showing reactions of mouse antisera to rBCLA purified antigen. The sera were collected from BALB/c mice 21 days post-intraperitoneal inoculation of 106 tachyzoites of 76K-GFP-luc WT or Δbcla strains. b The sera were collected from NMRI mice 22 months post-orally inoculation of 20 cysts of the type II (76K-GFP-luc) strain. c The sera were collected from BALB/c mice 42 days post-intraperitoneal inoculation of 103 to 105 tachyzoites of the type II (PruA7) strain. The sera are reacting quite proportionally with rBCLA according to the tachyzoite load. d The sera were collected from CBA mice 80 days post-intraperitoneal inoculation of 106 tachyzoites of the type II (ME49) strain. e, f IgG immunoblot showing reactions of mouse antisera to rBCLA purified antigen (e). The sera were collected from NMRI mice 42 days post-intraperitoneal inoculation of 106 type III (CTG) tachyzoites. The serological status of the infected mice was determined by immunoblot using the LDBio Diagnostics test (f). g The sera were collected from CBA mice 7 days post-intraperitoneal inoculation of 103 tachyzoites of the type I (RH) strain. h The sera were collected from NMRI mice 7 days post-intraperitoneal inoculation of 104 tachyzoites of the haplotype 11 (COUG) strain. i The sera were collected from NMRI mice 42 days post-intraperitoneal inoculation of 106 tachyzoites of the attenuated type II (PruΔku80). The serological status of the infected mice was determined by immunoblot using the LDBio Diagnostics test (lower panels)