Fig. 1

Data flow describing the extraction and topological annotation of OR natural variants. Functional human OR genes were used as queries for genotype searches in gnomAD, and the results were stored in a mutation data table (left side of the diagram). BLAST searches were used to localize the corresponding OR UniProt sequences. Topological regions and BW notation were defined from a structure-based MSA with the OR sequences and class A GPCRs with solved 3D atomic coordinates (right side of the diagram). This information and their associated substitution scores were transferred to each entry in the mutation data table, thus completing the annotation process. Full-length sequences and accession numbers of the sequences used in the study are available in the supplementary information