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Fig. 4 | BMC Biology

Fig. 4

From: GRK2 regulates GLP-1R-mediated early phase insulin secretion in vivo

Fig. 4

GRK2 levels and activity can modulate β-arrestin 2 association. Western blot analysis of the level of GRK2 silencing in Min6B1 cells (siSc: siRNA Scrambled; siGRK2: siRNA αAdrbk1), n = 4 independent experiments (a). AUC of β-arrestin 2 or Gαs recruitment quantified by NanoBIT and nanoBRET, respectively, for 30 min after GLP-1R activation in Min6B1 cells silenced (b) or inhibited (c) for GRK2, n = 4 (b) or 3 (c) independent experiments, each point is an average from 3 to 4 technical replicas. Means ± SEM data are represented. Statistical analysis was performed by paired t test (a) or paired one-way ANOVA followed by Bonferroni’s post hoc test (b, c). *p < 0.05, **p < 0.01, ***p < 0.001

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