CDS | N-terminal taga | Solubilityb (%) | Yield (mg/L) | Reference |
---|
Native | His6 | N.R.e | 11 | [51] |
His8-28mer-His8 | 41 | 5 | [44] |
GeneArt (n = 5) | His6 | 38 | 23 ± 4 | This work |
DNA2.0 | His6 | N.R.e | 15 | [52] |
GeneWiz (opt)c | His8-28mer-His8 | 24 ± 3 | 15 ± 2 | [44] |
GeneWiz (KRasRARE)d | His8-28mer-His8 | 52 ± 22 | 27 ± 11 | [44] |
- aAll N-terminal tags were reported to be followed by a TEV protease cleavage site except the GeneArt sequence that contains a dual Thrombin-TEV cleavage site
- bSolubility measurements represent the normalized ratio of overexpressed protein for the total protein and soluble extract lanes observed in SDS-PAGE analysis
- cAverage and standard deviation for reported values with 0.1–1.0 mM IPTG
- dKRasRARE represents the average and standard deviation for reported values of the GeneWiz optimized sequence w/ either a single or multiple reintroduced rare codons (I46, I84, R164, I84/R164, and I46/I84/R123/R164)
- eN.R. not reported