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Table 3 Reported solubility and yield of KRas4B (1–169) from expression in E. coli

From: Assessing optimal: inequalities in codon optimization algorithms

CDS

N-terminal taga

Solubilityb (%)

Yield (mg/L)

Reference

Native

His6

N.R.e

11

[51]

His8-28mer-His8

41

5

[44]

GeneArt (n = 5)

His6

38

23 ± 4

This work

DNA2.0

His6

N.R.e

15

[52]

GeneWiz (opt)c

His8-28mer-His8

24 ± 3

15 ± 2

[44]

GeneWiz (KRasRARE)d

His8-28mer-His8

52 ± 22

27 ± 11

[44]

  1. aAll N-terminal tags were reported to be followed by a TEV protease cleavage site except the GeneArt sequence that contains a dual Thrombin-TEV cleavage site
  2. bSolubility measurements represent the normalized ratio of overexpressed protein for the total protein and soluble extract lanes observed in SDS-PAGE analysis
  3. cAverage and standard deviation for reported values with 0.1–1.0 mM IPTG
  4. dKRasRARE represents the average and standard deviation for reported values of the GeneWiz optimized sequence w/ either a single or multiple reintroduced rare codons (I46, I84, R164, I84/R164, and I46/I84/R123/R164)
  5. eN.R. not reported