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Table 4 Variables that are considered for optimized DNA sequences applied to the KRas4B CDS: Median codon frequency, median value for the violin plots shown in Fig. 3; mean frequency, average frequency for the violin plots shown in Fig. 3; CAI, Codon Adaptation Index; %GC, average percent GC content in a DNA sequence; %GC3, average percent GC content in the third (wobble) codon position; %Min window, percent of the DNA sequence that exists with a minimum below zero in the MinMax profile

From: Assessing optimal: inequalities in codon optimization algorithms

Vendor algorithm

Median frequency

Mean frequency

CAI

%GC

%GC3

%Min window

Native DNA

0.339

0.370

0.61

37.7

30.2

65

IDT

0.373

0.391

0.68

45.1

50.0

27

Twist

0.373

0.399

0.70

51.5

67.0

17

DNA 2.0a

0.404

0.431

0.69

51.8

63.0

0

GeneWiz

0.418

0.446

0.79

54.0

73.6

7

Genscript

0.425

0.458

0.82

54.0

73.6

6

JCAT

0.429

0.470

0.84

46.4

50.0

0

OPTIMIZER

0.429

0.479

0.87

48.0

54.9

0

GeneArt

0.495

0.496

0.88

42.5

37.9

0

COOL

0.509

0.529

0.97

48.1

55.5

0

  1. aReference [52]