Fig. 2

FLIC current is aversive to flies. a Turning on FLIC current (10-MΩ resistor) significantly reduces food intake of 18-h food-deprived flies, as measured by fluorescent dye ingestion (FU, fluorescence units measured in plate reader; ***p < 0.0001, n = 33, 34, 51, 49 from 12 FLIC arenas; an n of 1 is the combined fluorescence from 2 flies). b Redesigned FLIC with adjustable current (FLEA) shows a decrease in food intake as a function of current (p < 0.0001; one-way Kruskal-Wallis ANOVA, n = 39, 32, 36, 28, 29 from 11 to 12 arenas, 2 flies per n). c Food interaction time, as measured by the FLEA current, is also significantly reduced as the current increases (p < 0.0001; n = 11, 12, 12, 12 arenas with 10 males each; NA, not available; 18 h deprivation, 400 mM sucrose in b and c). d Flies change their interaction time preference as the current increases in one of two equal-sucrose wells (p < 0.0001; n = 10, 11, 11, 10 arenas with 10 males each)