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Table 2 Strains and plasmids used in this study

From: Natural combinatorial genetics and prolific polyamine production enable siderophore diversification in Serratia plymuthica

Strain and genotype

Phenotype

Strain collection number

Reference

S. plymuthica V4

Wild-type

ZK4911

[16]

S. plymuthica V4 schE::GntR

Catecholate siderophore deficient

ZK4952

S. plymuthica V4 schF0::GntR

Enterobactin deficient

ZK4962

S. plymuthica V4 schF3::GntR

Polyamine-containing siderophore deficient

ZK4987

S. plymuthica V4 schH::GntR

Polyamine-containing siderophore deficient

ZK4984

S. plymuthica V4 schI::GntR

Aerobactin deficient

SA921

This study

S. plymuthica V4 schF0::GntR pTrc99A_schF0

S. plymuthica V4 schF0::GntR carrying pTrc99A_schF0

SA956

This study

S. plymuthica V4 schF0::GntR pTrc99A

S. plymuthica V4 schF0::GntR carrying pTrc99A

SA958

This study

S. plymuthica V4 pTRC99

S. plymuthica carrying pTRC99_schF0

SA960

This study

S. plymuthica V4 sch_13190::GntR

DAP defective; Sch_131390 mutant

SA976

This study

S. plymuthica V4 sch_13195::GntR

DAP defective; Sch_131395 mutant

SA977

This study

S. plymuthica V4 sch_20905::GntR

Cadaverine defective; Sch_20905 mutant

SA970

This study

S. plymuthica V4 sch_21940::GntR

Putrescine defective; Sch_21940 mutant

SA974

This study

S. plymuthica V4 sch_21945::GntR

Putrescine defective; Sch_21945 mutant

SA975

This study

S. plymuthica V4 sch_22085::GntR

Spermidine defective; Sch_22085 mutant

SA972

This study

S. plymuthica V4 sch_22090::GntR

Spermidine defective; Sch_22090 mutant

SA971

This study

S. plymuthica V4 sch_22290::GntR

Putrescine defective; Sch_22290 mutant

SA973

This study

Plasmid

Genotype

Reference number

Reference

pBTK30

R6K ori and Genr cassette

–

[98]

pSC30A

pBTK30 with a 600-bp fragment of schI cloned between StuI and SpeI, which replaces Mariner C9 and Ampr

SA918

This study

pTrc99A

IPTG-inducible expression vector, Ampr

–

[99]

pTrc99A_schF0

pTrc99A carrying schF0 between NcoI and XbaI

SA956

This study