Strain and genotype | Phenotype | Strain collection number | Reference |
---|---|---|---|
S. plymuthica V4 | Wild-type | ZK4911 | [16] |
S. plymuthica V4 schE::GntR | Catecholate siderophore deficient | ZK4952 | |
S. plymuthica V4 schF0::GntR | Enterobactin deficient | ZK4962 | |
S. plymuthica V4 schF3::GntR | Polyamine-containing siderophore deficient | ZK4987 | |
S. plymuthica V4 schH::GntR | Polyamine-containing siderophore deficient | ZK4984 | |
S. plymuthica V4 schI::GntR | Aerobactin deficient | SA921 | This study |
S. plymuthica V4 schF0::GntR pTrc99A_schF0 | S. plymuthica V4 schF0::GntR carrying pTrc99A_schF0 | SA956 | This study |
S. plymuthica V4 schF0::GntR pTrc99A | S. plymuthica V4 schF0::GntR carrying pTrc99A | SA958 | This study |
S. plymuthica V4 pTRC99 | S. plymuthica carrying pTRC99_schF0 | SA960 | This study |
S. plymuthica V4 sch_13190::GntR | DAP defective; Sch_131390 mutant | SA976 | This study |
S. plymuthica V4 sch_13195::GntR | DAP defective; Sch_131395 mutant | SA977 | This study |
S. plymuthica V4 sch_20905::GntR | Cadaverine defective; Sch_20905 mutant | SA970 | This study |
S. plymuthica V4 sch_21940::GntR | Putrescine defective; Sch_21940 mutant | SA974 | This study |
S. plymuthica V4 sch_21945::GntR | Putrescine defective; Sch_21945 mutant | SA975 | This study |
S. plymuthica V4 sch_22085::GntR | Spermidine defective; Sch_22085 mutant | SA972 | This study |
S. plymuthica V4 sch_22090::GntR | Spermidine defective; Sch_22090 mutant | SA971 | This study |
S. plymuthica V4 sch_22290::GntR | Putrescine defective; Sch_22290 mutant | SA973 | This study |
Plasmid | Genotype | Reference number | Reference |
pBTK30 | R6K ori and Genr cassette | – | [98] |
pSC30A | pBTK30 with a 600-bp fragment of schI cloned between StuI and SpeI, which replaces Mariner C9 and Ampr | SA918 | This study |
pTrc99A | IPTG-inducible expression vector, Ampr | – | [99] |
pTrc99A_schF0 | pTrc99A carrying schF0 between NcoI and XbaI | SA956 | This study |