Fig. 1
From: Single-cell morphological characterization of CRH neurons throughout the whole mouse brain
Comparison of the morphological features of CRH neurons in three fluorescent-reporter mouse lines. Comparisons of the distributions and morphologies of fluorescent-labeled CRH neurons in several brain regions of CRH-IRES-Cre;Ai6, CRH-IRES-Cre;Ai14, and CRH-IRES-Cre;Ai32 mice in the OB (A–C), cortex (D–F), and PVN (G–I). (A–C, a–c) The dotted boxes show ZsGreen1 (a), td-Tomato (b), and EYFP (c) labeling of synaptic globular structures in the Gl of the three mouse lines. A–B (a’–b’) The dotted boxes and magnified images show ZsGreen1 (a’) and td-Tomato (b’) labeling of cell bodies and dendrites (indicated by the arrowheads) in the Mi and EPl of CRH-IRES-Cre;Ai6 and CRH-IRES-Cre;Ai14 mice. C (c’) The dotted box and magnified image show EYFP-labeled cell body (indicated by the arrowhead) and dendrites in a CRH-IRES-Cre;Ai32 mouse. D–F (d–f) The dotted boxes and magnified images show ZsGreen1 (d), td-Tomato (e), and EYFP (f) labeling of cell bodies and dendrites in layer 1 and layer 2/3 of the three mouse lines. G ZsGreen1-labeled cell bodies in (dotted-curved box) and outside (arrowheads) of the PVN of CRH-IRES-Cre;Ai6 mice. H Td-Tomato-labeled cell bodies in the PVN (dotted-curved box) of CRH-IRES-Cre;Ai14 mice. I (i) EYFP-labeled cells (I, dotted-curved box) and fibers (i, dotted box and magnified image) in the PVN of CRH-IRES-Cre;Ai32 mice. Scale bars = 100 μm. J The difference in density of fluorescently labeled neurons in several brain regions of three reporter mouse lines. Data are shown as mean ± SEM; n = 4 mice from independent experiments, 4 slices per mouse