Fig. 3
From: Single-cell morphological characterization of CRH neurons throughout the whole mouse brain
Three-dimensional distributions and single-cell reconstructions of CRH neurons in several brain regions. a–h Original images (left half) and reconstructions (right half) of CRH neurons in different brain regions, including the OB (a), LSD (b), BST (c), CeA (d), VMPO (e), Hip (f), SCN (g), and DC (h). The original images were inverted into grayscale images. The reconstructed somata and dendrites of neurons are indicated by purple bodies and red lines, respectively. i Typical reconstructed neurons show the common bipolar morphology found in different brain regions, including the mPFC, BST, VMPO, PaAp, Pe, and SCN. j Somatic volumes of CRH neurons in different brain regions [one-way ANOVA, P < 0.0001, F (8, 125) = 23.24], number in the bars indicate the number of neurons calculated. k Total fibrous lengths of CRH neurons in different brain regions [one-way ANOVA, P < 0.0001, F (8, 90) = 13.93]. l Numbers of fibrous branches of CRH neurons in different brain regions [one-way ANOVA, P < 0.0001, F (8, 96) = 26.94]. m Sholl analysis of dendrites of neurons in the LSD, BST, and VMPO illustrate changes in the mean number of intersections with increasing radial distance from the soma, n = 16 cells for LSD, 7 cells for BST and 10 cells for VMPO. n Correlation between dendritic length and somatic volume in LSD neurons (Pearson’s correlation coefficient r = 0.68, P = 0.0029). o Correlation between dendritic length and somatic volume in VMPO neurons (Pearson’s correlation coefficient r = 0.88, P = 0.0019)