Fig. 2

Phylogenetic tree of the Stomoxys calcitrans OBPs with those of Drosophila melanogaster and Musca domestica and locations of OBPs on Stomoxys scaffolds. a Maximum likelihood phylogeny was constructed using the web server version of IQ-TREE software ([114]; best-fit substitution model, branch support assessed with 1000 replicates of UFBoot bootstrap approximation). The full phylogenetic tree can be found in Additional file 1: Figure S8. The S. calcitrans and M. domestica lineages are in green and blue, respectively, while D. melanogaster lineages are in mustard. Clades that are expanded in the muscids relative to Drosophila or lost in Drosophila are shaded in orange and gray. Purple shading comprises a clade of muscid OBPs with no apparent ortholog in Drosophila, while blue shading comprises a clade that includes the Drosophila Minus-C OBP subfamily and represents an apparent muscid expansion relative to DmelObp99c. b Three Stomoxys scaffolds on which 51 of 90 OBPs are organized. The location of each OBP gene is indicated by a horizontal line. Transcriptional directions are indicated by (+) for same direction as the scaffold or (−) for the opposite direction. The color of the box reflects the shaded clades in a