Fig. 9

Phylogenetic analysis of cytochrome P450 genes from Stomoxys calcitrans. Amino acid sequences from each family were aligned with the MUSCLE algorithm [201], and the alignments trimmed with the trimAl tool using the –strictplus option [266]. The trimmed alignment was used to construct a maximum likelihood phylogeny, rooted with Mus musculus CYP51 as the outgroup, with the web server version of IQ-TREE software (best-fit substitution model, branch support assessed with 1000 replicates of UFBoot bootstrap approximation; bootstrap percentages reported [114]). The CYP clades are identified by different colored lineages, and CYP gene clusters that are found in tandem within the genome are shaded in gray. P450 gene names were assigned based on comparative analyses (see Additional file 13), and the full phylogenetic tree can be found in Additional file 1: Figure S21