Fig. 3

Dynamic regulation of switch-Cyclop1 (C1) activity by different light colours. a After 30 s UV-A (380 nm, 9.6 μW/mm²) illumination, cGMP productions were measured at different time points under dark or constant illumination with light of different wavelengths. Light intensities of blue (473 nm), green (532 nm), orange (593 nm), and red (635 nm) were all adjusted to 4.8 μW/mm². n = 3, all data points were shown. b Dynamic control of switch-Cyclop1 activity by UV-A and blue light; 30 s UV-A light (380 nm, 9.6 μW/mm²) and 30 s blue (473 nm, 9.6 μW/mm²) illumination were used to activate and inhibit cGMP production. , 30 s UV-A at time 0, then dark, 30 s blue at 4 min, then dark, then 30 s UV-A at 12 min and then dark until 18 min; , 30 s UV-A at time 0, then dark, then 30 s blue at 4 min and then dark until 18 min; , constant blue until 18 min; , constant dark until 18 min. Measurements of cGMP production in the dark or under constant blue illumination were performed in parallel. For both a and b, 30 ng cRNA were injected; measurements were done 3 dpi. The results were referring to the total activities of membrane proteins extracted from one oocyte. n = 3; all data points were shown