Fig. 4
From: An engineered membrane-bound guanylyl cyclase with light-switchable activity
Illumination conditions required for switch-Cyclop1 regulation. a Enzyme activities after 30 s illumination with UV-A light of different intensities (0, 0.6, 1.2, 2.4, 4.8, 9.6 μW/mm2). A data point with 30 s 9.6 μW/mm2 UV-A illumination was set to “1” for normalization. Curve fitted with Hill1 equation. b Enzyme activities after different time (0, 1, 2, 4, 8, and 16 min) of illumination with 0.6 μW/mm2 UV-A. A data point with 8 min 0.6 μW/mm2 UV-A illumination was set to “1” for normalization. Curve fitted with Hill1 equation. c After 30 s 9.6 μW/mm2 UV-A (380 nm) light stimulation, 30 s blue light (473 nm) illuminations with different intensities (0.3, 0.6, 1.2, 2.4, 4.8, 9.6 μW/mm2) were used to inhibit the evoked GC activity. Curve fitted with Hill1 equation. d After 30 s 9.6 μW/mm2 UV-A (380 nm) light stimulation, 0.6 μW/mm2 blue light (473 nm) illumination of different time (0.25, 0.5, 1, 2, 4, and 8 min) were used to inhibit the evoked GC activity. Curve fitted with Hill1 equation. For c and d, one repeat of enzyme activity after 30 s UV-A light (380 nm, 9.6 μW/mm2) was set to “1” for normalization; 30 ng cRNA were injected for 3 days’ expression. For all, n = 6, all data points were shown