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Fig. 5 | BMC Biology

Fig. 5

From: An engineered membrane-bound guanylyl cyclase with light-switchable activity

Fig. 5

switch-Cyclop1 and Cr2c-Cyclop activities under different light conditions. Enzyme activities of switch-Cyclop1 (a) and Cr2c-Cyclop1 (b) under different light conditions. Reactions are done in the dark or under constant light illumination conditions. Illumination is performed with 380 nm UV-A and 505 nm cyan LED. Exact illumination conditions are UV-1 (UV-A 1 μW/mm2), Cyan-4 (cyan light 4 μW/mm2), UV/Cy-1/0.5 (UV-A 1 μW/mm2 + cyan 0.5 μW/mm2), UV/Cy-1/1 (UV-A 1 μW/mm2 + cyan 1 μW/mm2), UV/Cy-1/2 (UV-A 1 μW/mm2 + cyan 2 μW/mm2) and UV/Cy-1/4 (UV-A 1 μW/mm2 + cyan 4 μW/mm2); 40 ng cRNA were injected for each; measurements were done 3 dpi. The results were referring to the total activities of membrane proteins extracted from one oocyte. n = 6, error bars = SD

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