Fig. 1

CRISPR/Cas9-mediated ik knock-out (KO) embryos display abnormal embryonic phenotypes and lethality. a Sequence alignment of IK proteins from human, mouse, zebrafish, chicken, and chimpanzee by NCBI COBALT. The conserved sequence is shown in red. b Schematic of zebrafish ik locus and CRISPR/Cas9 targeted region. The asterisk denotes the stop codon. c Genotype confirmation of ik in wild type (WT), heterozygous, KO embryos by RT-PCR analysis. WT alleles were digested with the BslI enzyme. +/+: 70 + 167 bp, +/−: 70 + 167 + 237 bp, −/−: 237 bp. d Relative mRNA levels of ik in WT and ik KO embryos at 1.5 and 4 days post-fertilization (dpf) using qRT-PCR analysis. The average of three independent experiments is shown with error bars. e Lateral views of WT and ik KO embryos during embryonic development. Scale bar = 250 μm