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Fig. 2 | BMC Biology

Fig. 2

From: The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization

Fig. 2

Supramolecular assemblies of CHMP2A+CHMP3 versus CHMP2B on GUVs. a Spinning disk images of supramolecular assemblies of CHMP2B-ΔC (called CHMP2B) in BP buffer on 10% PI(4,5)P2-GUVs. After 15-min incubation of the GUVs with the protein solution, CHMP2B-ΔC at high bulk protein concentration (1 μM) (first panel) fully covers the vesicle surface, whereas at lower protein concentration (500 nM), CHMP2B-ΔC assembles into a reticular-like network on the GUV (second panel). A z-projection of the whole GUV is shown. Scale bar, 10 μm. b Co-localization of Fluo-PI(4,5)P2 and CHMP2B-ΔC on GUVs. A z-projection of the upper part of the GUV is shown. Scale bar, 10 μm. c Spinning disk images of supramolecular assemblies of MBP-CHMP2A-ΔC (500 nM) + CHMP3 (2 μM) in BP buffer on 10% PI(4,5)P2-GUVs. MBP-CHMP2A-ΔC (called CHMP2A) fluorescent signal is displayed. After 60-min incubation, the co-polymer covers the vesicle surface in a homogeneous manner with the presence of some protrusions at the surface of the GUV (zoom-in). A z-projection is shown including a zoom-in in the right panel, showing short protrusions at the surface of the GUV. Scale bar, 10 μm

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