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Fig. 3 | BMC Biology

Fig. 3

From: The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization

Fig. 3

Mechanical properties of GUVs in the presence of CHMP2B versus CHMP2A+CHMP3 measured by micropipette aspiration. a Representative confocal single-plane images of micropipette aspiration performed at σ ≈ 5.10−3 N m−1 on a bare GUV containing 10% PI(4,5)P2- (first panel), and on GUVs coated with CHMP2B-ΔC alone (second panel), CHMP2B-ΔC + CHMP3 (fourth panel), and MBP-CHMP2A-ΔC + CHMP3 (fifth panel) (CHMP2B corresponds to CHMP2B-ΔC and CHMP2A to MBP-CHMP2A-ΔC). The occasional rupture of CHMP2B polymer at high tension (σ ≈ 10−3 N m−1) is shown (third panel). Scale bar, 10 μm. N = 30 per condition. b Percentage of aspirated GUVs at σ ≈ 10−3 N m−1 with formation of a tongue inside the micropipette. Comparison between CHMP2B-ΔC-only GUVs and GUVs coated with CHMP2B-ΔC +CHMP3. N = 14 per condition. c Characteristic curves of the variation of the excess area ΔA/A as a function of the applied tension for a bare GUV (magenta) and a GUV coated with MBP-CHMP2A-ΔC +CHMP3 proteins (blue). The linear fit of each curve is represented (black). d Box plot of the stretching modulus for bare GUV (N = 20 experiments; magenta) or in the presence of MBP-CHMP2A-ΔC+CHMP3 (N = 30 experiments; blue) interacting with the GUV membrane (p-value = 0.002)

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