Fig. 3.

BG-flow is suitable to detect BG4 signal changes in different cell cycle phases. a Histogram plot of the BG4 signal distribution in MCF-7 cells incubated with BG4 (black) or without antibody (white). b Histogram plot of the DAPI signal in MCF-7 cells. The cell cycle distribution was obtained dividing the cells per DAPI amount. The table states the quantification (% of total) of the cells in the three cell cycle phases. c Quantification of the BG4 signal distribution in MCF-7 cells divided per cell cycle phases. Results are based on the average of n = 3 biologically independent experiments. Significance was calculated based on the one-sided Student’s t-test. Asterisks indicate statistical significance in comparison with wildtype cells: **p < 0.01, ***p < 0.001. d Histogram plot of the DAPI signal in untreated THP-1 cells (white) and cells incubated 24 h with 50 μM PDS (black). The table states the cell cycle distribution obtained by analysis with the cell cycle tool in FlowJo. e Quantification of the FL1-BG4 signal distribution in untreated THP-1 cells (white) and cells incubated 24 h with 50 μM PDS (black) in the different cell cycle phase. The table states the BG4 signal increase in the different cell cycle phases. Results are based on the average of n = 3 biologically independent experiments. An excel file providing individual data values is available in the section “Availability of data and materials”